August Krogh Institute, University of Copenhagen Staff information

Peter Leth Jørgensen

List of publications

Key references for research areas A-D and articles from the last 5 years

A. Purification of the Na,K-pump from mammalian kidney

Peter L. Jørgensen and Jens C. Skou, Preparation of highly active Na,K-ATPase from the outer medulla of rabbit kidney. Biochem.Biophys.Res.Commun. 37: 39-46, 1969.

Peter L. Jørgensen and Jens C. Skou, Purification and characterization of Na,K-ATPase. I. The influence of detergents on the activity of Na,K-ATPase in preparations from the outer medulla of rabbit kidneys. Biochim.Biophys. Acta, 233: 366-380, 1971.

Peter L. Jørgensen, Purification and characterization of Na,K-ATPase. III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate. Biochim. Biophys. Acta, 356: 36-52, 1974.

Peter L. Jørgensen. Isolation and characterization of the components of the sodium pump. Quart.Rev. Biophys. 7: 239-274,1975.

Peter L. Jørgensen, Mechanism of the Na,K-ion pump. Protein structure and conformations of the pure Na,K-ATPase. BBA Reviews on Biomembranes. Biochim. Biophys. Acta 694: 27-68, 1982.

Peter L. Jørgensen, Isolation of Na,K-dependent ATPase. Methods Enzymol. 32: 277-290, 1974.

Peter L. Jørgensen, Purification of Na,K-ATPase; Enzyme sources and preparative problems. Update preparation of Na,K-ATPase from mammalian kidney. Methods Enzymol.156: 29-43, 1988. and Selected Methods Enzymol. Biomembranes, 1997, pp. 779-793.

B. Crystallization

Elisabeth Skriver, Arvid B. Maunsbach and Peter L. Jørgensen, Formation of two-dimensional crystals in pure membrane-bound Na,K-ATPase. FEBS Letters 131: 219-222, 1981.

Hans Hebert, Peter L. Jørgensen, Elisabeth Skriver and Arvid B. Maunsbach, Crystallization patterns of membrane-bound Na,K-ATPase. Biochimica et Biophysica Acta, 689: 571-574, 1982.

Peter L. Jørgensen, Elisabeth Skriver, Hans Hebert and Arvid B. Maunsbach. Structure of the Na,K-pump: Crystallization of pure membrane-bound Na,K-ATPase and identification of functional domains of the a- subunit. Annals of the New York Academy of Sciences, 402: 203-219, 1982.

C. Soluble Na,K-ATPase

Jaakko R. Brotherus, Lene Jacobsen and Peter L. Jørgensen. Soluble and enzymatically stable Na,K-ATPase from mammalian kidney consisting predominantly of ab-units. Preparation, assay and reconstitution of active transport. Biochimica et Biophysica Acta, 731: 290-303, 1983.

Peter L. Jørgensen and Jens P. Andersen. Thermoinactivation and aggregation of ab-units in soluble and membrane-bound Na,K-ATPase. Biochemistry, 25: 2889-2897, 1986.

D. Protein Conformations of Na,K-ATPase

Peter L. Jørgensen, Purification and characterization of Na,K-ATPase. V. Conformational changes in the enzyme. Transitions between the Na-form and the K-form studied with tryptic digestion as a tool. Biochimica et Biophysica Acta, 401: 399-415, 1975.

Peter L. Jørgensen, Purification and characterization of Na,K-ATPase. VI. Differential tryptic modification of catalytic functions of the purified enzyme in presence of NaCl and KCl. Biochimica et Biophysica Acta, 466: 97-108, 1977.

Peter L. Jørgensen and Janne Petersen, Chymotryptic cleavage of a-subunit in E1-forms of renal Na,K-ATPase: effects on enzymatic properties, ligand binding and cation exchange. Biochimica et Biophysica Acta, 821: 319-333, 1985.

Jens P. Andersen and Peter L. Jørgensen, Conformational states of sarcoplasmic reticulum Ca-ATPase as studied by proteolytic cleavage. Journal of Membrane Biology, 88: 187-198, 1985.

Peter L. Jørgensen. Conformational transitions in the Na,K-pump. In: The Sodium Pump. Rockefeller University Press, 1991, pp. 189-200

Articles from the last 5 years, Peter L. Jørgensen, 1996-2001

Dan A. Klærke, Hubert Wiener, Thomas Zeuthen and Peter L. Jørgensen. Regulation of Ca2+-activated K+ channel from rabbit distal colon epithelium by phosphorylation and dephosphorylation. Journal of Membrane Biology, 151: 11-18, 1996.

Per A. Pedersen, Jakob H. Rasmussen and Peter L. Jørgensen. Expression in high yield of pig a1b1 Na,K-ATPase and inactive mutants D369N and D807N in Saccharomyces cerevisiae. Journal of Biological Chemistry, 271: 2514-2522, 1996.

Karl Lücking, Jesper M. Nielsen, Per Amstrup Pedersen and Peter l. Jørgensen. Na,K-ATPase isoform (a3, a2, a1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding. American Journal of Physiology 271: F253-F260, 1996.

Per A. Pedersen, Jakob H. Rasmussen and Peter L. Jørgensen. Consequences of mutations to the phosphorylation site of a-subunit of Na,K-ATPase for ATP binding and E1-E2 conformational equilibrium. Biochemistry, 35: 16085-16093, 1996.

Peter L. Jørgensen, Jakob H. Rasmussen, Jesper M. Nielsen, and Per Amstrup Pedersen. Transport linked conformational changes in Na,K-ATPase: Structure-function relationships of ligand binding and E1-E2 conformational transitions. Annals of the New York Academy of Sciences, 834: 161-175, 1997.

Per A. Pedersen, Jakob H. Rasmussen and Peter L. Jørgensen. Phosphorylation site of a-subunit of Na,K-ATPase for ATP binding and E1-E2 conformational equilibrium. Annals of the New York Academy of Sciences, 834: 454-457, 1997.

Jakob H. Rasmussen, Per A. Pedersen, and Peter L. Jørgensen. High yield fermentation of Na,K-ATPase in yeast. Annals of the New York Academy of Sciences, 834: 469-472, 1997.

Per A. Pedersen, Jakob H. Rasmussen, Jesper M. Nielsen and Peter L. Jørgensen. Identification of Asp804 and Asp808 as Na+ and K+ coordinating residues in a-subunit of Na,K-ATPase. FEBS Letters, 400: 206-210, 1997.

Peter L. Jørgensen. Purification of Na,K-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney. Biomembranes, Selected Methods in Enzymology Series, 779-794, 1997.

Peter L. Jørgensen. Nobelprisen 1997 i kemi til professor Jens Chr. Skou, Aarhus Universitet. Statusartikel. Ugeskrift for læger, 159: 7639-7644, 1997.

Jesper M. Nielsen Per A. Pedersen, Steven J.D. Karlish and Peter L. Jørgensen. Importance of Intramembrane Carboxylic Acids for Occlusion of K+ Ions at equilibrium in renal Na,K-ATPase, Biochemistry, 37: 1961-1968, 1998

Peter L. Jørgensen, Jesper M. Nielsen, Jakob H. Rasmussen and Per A. Pedersen. Structure-Function Relationships based on ATP Binding and Cation Occlusion at Equilibrium in Na, K-ATPase. (Astra-Hässle, Cation Pump Symposium). Acta Physiol. Scand. 163, Suppl. 643: 79-87, 1998.

Peter L. Jørgensen, Jesper M. Nielsen, Jakob H. Rasmussen and Per Amstrup Pedersen: Structure function relationships of E1-E2 transitions and cation binding in Na,K-pump protein. (EBEC issue). Biochimica et Biophysica Acta, Bioenergetics, 1365: 65-70, 1998.

Pedersen, P.A., Nielsen, J.M., Rasmussen, J.H., Jørgensen, P.L. Contribution to Tl+, K+, and Na+ binding of Asn776, Ser775, Thr774, Thr772 and Tyr771 in cytoplasmic part of fifth transmembrane segment in a--subunit of renal Na,K-ATPase.  Biochemistry, 37: 17818-17827, 1998.

P.L. Jørgensen, J.M. Nielsen, J.H. Rasmussen, and P.A. Pedersen. Identification of amino acid residues involved in Na+ or K+ (Tl+) binding in Na,K-ATPase; contributions to Na+/K+ specificity. Excerpta Medica Int. Congress Series 1207. Na/K-ATPase and Related ATPases. pp. 95-102, 2000.

P. L. Jørgensen, J. M. Nielsen, J. H. Rasmussen, and P. A. Pedersen: Structure and function of Na+ or K+ (Tl+) binding sites in Na,K-ATPase. Excerpta Medica. Int. Congress Series 1208. Control and Diseases of Sodium Dependent Transport Proteins and ion Channels.pp. 1-4, 2000.

 

Per A. Pedersen, Jesper R. Jørgensen and Peter Leth Jørgensen. Importance of Conserved a- subunit Segment 709-GDGVND for Mg2+ binding, Phosphorylation, and Energy Transduction in Na,K-ATPase. Journal of Biological Chemistry, 275: 37588-37595, 2000. 

Published on September 11, 2000 as Manuscript M005610200.

 

Peter L. Jørgensen: Aspects of Gene Structure and Functional Regulation of the Isozymes of Na,K-ATPase. Cell Mol Biol (Noisy-le-grand).  Mar;47(2):231-8, 2001.

Peter L. Jørgensen and Per A. Pedersen: Structure-Function Relationships of Na+ , K+ , ATP, or Mg2+ Binding and Energy Transduction in Na,K-ATPase. BBA Reviews on Bioenergetics, Biochim Biophys Acta.Review. 1505(1):57-74, 2001. 

Peter L. Jørgensen, Jesper R. Jørgensen and Per A. Pedersen: Role of Conserved TGDGVND-Loop in Mg2+ Binding, Phosphorylation, and Energy Transfer in Na,K-ATPase. Journal of Bioenergetics and Biomembranes, 33: 367-377, 2001 

 

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